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E1014
Sigma
Benzonase® Nuclease
≥250 units/μL, ≥90% (SDS-PAGE), recombinant, expressed in Escherichia coli, buffered aqueous glycerol solution
| Synonym: | Endonuclease from Serratia marcescens |
| CAS Number: | 9025-65-4 |
| Enzyme Commission (EC) Number: | 3.1.30.2 ( BRENDA | IUBMB ) |
| EC Number: | 231-791-2 |
| MDL number: | MFCD00131010 |
Description
| Application | Used for the removal of nucleic acid from protein samples. |
| Biochem/physiol Actions | Digests native or heat-denatured DNA and RNA. |
| Legal Information | Benzonase® Nuclease is manufactured by Merck KGaA and its affiliates. The technology is covered by US Patent 5,173,418 and EP Patent 0,229,866. Nycomed Pharma A/S (Denmark) claims worldwide patent rights to Benzonase® Nuclease, which are licensed exclusively to Merck KGaA, Darmstadt, Germany. Benzonase® is a registered trademark of Merck KGaA, Darmstadt, Germany. |
| Unit Definition | One unit will digest sonicated salmon sperm DNA to acid-soluble oligonucleotides equivalent to a ΔA260 of 1.0 in 30 min at pH 8.0 at 37 °C (reaction volume 2.625 ml). |
| Physical form | Solution in 50% glycerol containing 20 mM Tris HCl, pH 8.0, 2 mM MgCl2, and 20 mM NaCl. |
| Benzonase is a registered trademark of Merck KGaA |
Properties
| recombinant | expressed in Escherichia coli |
| assay | ≥90% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| concentration | ≥250 units/μL |
| foreign activity | protease, essentially free |
| storage temp. | −20°C |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, half-mask respirator (EU), half-mask respirator (US), multi-purpose combination respirator cartridge (US) |
| WGK Germany | 1 |
References
| reference | Molloy, M.P., et al. Electrophoresis 19, 837-844, (1998) |
| Herbert, B.R. Electrophoresis 19, 845-851, (1998) |
